Find the serial number for your Nest Thermostat. On the box, look for S/N and find the number that follows it. You can also find it in the thermostat technical menu. Find the serial number for your Stadia controller. You can find the serial number on the back of the controller under the G logo. SerialEM install packages are available in three forms: The 32-bit version can run on any system but can access only 2-3 GB of memory. The 64-bit version can use an unlimited amount of memory but requires a 64-bit operating system. It will not work with a JEOL microscope unless the 64-bit version of TemExternal (TemExt64.dll) is installed. Dubbed the 'Night Stalker,' Richard Ramirez was an American serial killer who broke into California homes, raping and torturing more than 25 victims and killing at least 13 over a two-year rampage.
This section is not a recipe for your experiment. It explains someprinciples for designing dilutions that give optimal results. Onceyou understand these principles, you will be better able to designthe dilutions you need for each specific case.
Often in experimental work, you need to cover a range ofconcentrations, so you need to make a bunch of differentdilutions. For example, you need to do such dilutions of thestandard IgG to make the standard curve in ELISA, and then againfor the unknown samples in ELISA.
You might think it would be good to dilute 1/2, 1/3, 1/10, 1/100.These seem like nice numbers. There are two problems with this series ofdilutions.
- The dilutions are unnecessarily complicated to make. You need to do a differentcalculation, and measure different volumes, for each one. It takes a longtime, and it is too easy to make a mistake.
- The dilutions cover the range from 1/2 to 1/100 unevenly.In fact, the 1/2 vs. 1/3 dilutions differ by only 1.5-fold in concentration,while the 1/10 vs. 1/100 dilutions differ by ten-fold. If you are going tomeasure results for four dilutions, it is a waste of time and materialsto make two of them almost the same. And what if the half-maximal signaloccurs between 1/10 and 1/100? You won't be able to tell exactly where itis because of the big space between those two.
Serial dilutions are much easier to make and they cover the range evenly.
Serial dilutions are made by making the same dilution step over and over,using the previous dilution as the input to the next dilution in each step.Since the dilution-fold is the same in each step, the dilutionsare a geometric series (constant ratio between any adjacent dilutions).For example:
- 1/3, 1/9, 1/27, 1/81
- 1/5, 1/25, 1/125, 1/625
When you need to cover several factors of ten (several 'orders of magnitude') witha series of dilutions, it usually makes the most sense to plot the dilutions(relative concentrations) on a logarithmic scale. This avoids bunching mostof the points up at one end and having just the last point way fardown the scale.
Before making serial dilutions, you need to make rough estimatesof the concentrationsin your unknowns, and your uncertainty in those estimates. For example,if A280 says you have 7.0 mg total protein/ml, and you thinkthe protein could be anywhere between 10% and 100% pure, then yourassay needs to be able to see anything between 0.7 and 7 mg/ml.That means you need to cover a ten-fold range of dilutions, or maybe a bitmore to be sure.
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If the half-max of your assay occurs atabout 0.5mg/ml,then your minimum dilution fold is(700mg/ml)/(0.5mg/ml) = 1,400.Your maximum is(7000mg/ml)/(0.5mg/ml) = 14,000.So to be safe, you might want to cover 1,000 through 20,000.
In general, before designing a dilution series, you need to decide:
- What are the lowest and highest concentrations (or dilutions)you need to test in order to be certain of finding the half-max? Thesedetermine the range of the dilution series.
- How many tests do you want to make? This determines the size of theexperiment, and how much of your reagents you consume. More tests will coverthe range in more detail, but may take too long to perform (or cost too much).Fewer tests are easier to do, but may not cover the range in enough detailto get an accurate result.
- What volume of each dilution do you need to make in order to haveenough for the replicate tests you plan to do?
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Now suppose you decide that six tests will be adequate (perhapseach in quadruplicate).Well, starting at 1/1,000, you need five equal dilution steps (giving yousix total dilutions counting the starting 1/1,000) that end ina 20-fold higher dilution (giving 1/20,000). You can decide on a goodstep size easily by trial and error. Would 2-fold work? 1/2, 1/4, 1/8, 1/16, 1/32. Yes, in factthat covers 32-fold, more than the 20-fold range we need. (The exact answeris the 5th root of 20, which your calculator will tell you is 1.82 foldper step. It is much easier to go with 2-fold dilutions and gives about thesame result.)
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So, you need to make a 1/1,000 dilution to start with. Then you need toserially dilute that 2-fold per step in five steps. You could make 1/1,000 byadding 1 microliter of sample to 0.999 ml diluent. Why is that a poor choice?Because you can't measure 1 microliter (or even 10 microliters) accuratelywith ordinary pipeters. So, make three serial 1/10 dilutions(0.1 ml [100 microliters] into 0.9 ml): 1/10 x 1/10 x 1/10 = 1/1,000.
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Now you could add 1.0 ml of the starting 1/1,000 dilution to1.0 ml of diluent, making a 2-fold dilution (giving 1/2,000).Then remove 1.0 ml from that dilution (leaving 1.0 ml for yourtests), and add it to 1.0 ml of diluent in the next tube (giving1/4,000). And so forth for 3 more serial dilution steps (giving1/8,000, 1/16,000, and 1/32,000). You end up with 1.0 ml of each dilution.If that is enough to perform all of your tests, this dilution planwill work. If you need larger volumes, increase the volumes you useto make your dilutions (e.g. 2.0 ml + 2.0 ml in each step).
Start studying econ 101 midterm. Learn vocabulary, terms, and more with flashcards, games, and other study tools. ECON101 MidTerm - Part 1 of 1 Question 1 of 20 Economics is the study of 100.0 Points 4.45 Points A.increasing the level of productive resources so Course Hero ECON101 MidTerm - Part 1 of 1 Question 1 of 20 Economics is. School American Public University Course Title ECON 101. Economics can be defined as the study of: How scarce resources are allocated to best meet society's goals. A production-possibilities curve indicates the. Econ 101 Midterm #1. ECON 224 First Exam. Chapter 9 and 10. Econ 101 midterm. View ECON101, Midterm, AMU.pdf from ECON 101 at American Military University. Quiz Submissions - Midterm Attempt 1 Written: Oct 2, 2020 6:28 PM - Oct 2, 2020 8:12 PM Submission View Your quiz has.